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Luis Miguel Goitizolo

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RE: ARE WE NOW IN THE END TIMES?
5/19/2016 5:41:59 PM

In Search For Cures, Scientists Create Embryos That Are Both Animal And Human

Heard on All Things Considered

Jeannie Phan for NPR

A handful of scientists around the United States are trying to do something that some people find disturbing: make embryos that are part human, part animal.

The researchers hope these embryos, known as chimeras, could eventually help save the lives of people with a wide range of diseases.

One way would be to use chimera embryos to create better animal models to study how human diseases happen and how they progress.

Perhaps the boldest hope is to create farm animals that have human organs that could be transplanted into terminally ill patients.

But some scientists and bioethicists worry the creation of these interspecies embryos crosses the line. "You're getting into unsettling ground that I think is damaging to our sense of humanity," says Stuart Newman, a professor of cell biology and anatomy at the New York Medical College.

The experiments are so sensitive that the National Institutes of Health has imposed amoratorium on funding them while officials explore the ethical issues they raise.

Nevertheless, a small number of researchers are pursuing the work with alternative funding. They hope the results will persuade the NIH to lift the moratorium.

"We're not trying to make a chimera just because we want to see some kind of monstrous creature," says Pablo Ross, a reproductive biologist at the University of California, Davis. "We're doing this for a biomedical purpose."

The NIH is expected to announce soon how it plans to handle requests for funding.

Recently, Ross agreed to let me visit his lab for an unusual look at his research. During the visit, Ross demonstrated how he is trying to create a pancreas that theoretically could be transplanted into a patient with diabetes.

The first step involves using new gene-editing techniques to remove the gene that pig embryos need to make a pancreas.

Working under an elaborate microscope, Ross makes a small hole in the embryo's outer membrane with a laser. Next, he injects a molecule synthesized in the laboratory to home in and delete the pancreas gene inside. (In separate experiments, he has done this to sheep embryos, too.)

After the embryos have had their DNA edited this way, Ross creates another hole in the membrane so he can inject human induced pluripotent stem cells, or iPS for short, into the pig embryos.

Like human embryonic stem cells, iPS cells can turn into any kind of cell or tissue in the body. The researchers' hope is that the human stem cells will take advantage of the void in the embryo to start forming a human pancreas.

Because iPS cells can be made from any adult's skin cells, any organs they form would match the patient who needs the transplant, vastly reducing the risk that the body would reject the new organ.

But for the embryo to develop and produce an organ, Ross has to put the chimera embryos into the wombs of adult pigs. That involves a surgical procedure, which is performed in a large operating room across the street from Ross's lab.

Pablo Ross of the University of California, Davis inserts human stem cells into a pig embryo as part of experiments to create chimeric embryos.

Rob Stein/NPR


The day Ross opened his lab to me, a surgical team was anesthetizing an adult female pig so surgeons could make an incision to get access to its uterus.

Ross then rushed over with a special syringe filled with chimera embryos. He injected 25 embryos into each side of the animal's uterus. The procedure took about an hour. He repeated the process on a second pig.

Every time Ross does this, he then waits a few weeks to allow the embryos to develop to their 28th day — a time when primitive structures such as organs start to form.

Ross then retrieves the chimeric embryos to dissect them so he can see what the human stem cells are doing inside. He examines whether the human stem cells have started to form a pancreas, and whether they have begun making any other types of tissues.

The uncertainty is part of what makes the work so controversial. Ross and other scientists conducting these experiments can't know exactly where the human stem cells will go. Ross hopes they'll only grow a human pancreas. But they could go elsewhere, such as to the brain.

"If you have pigs with partly human brains you would have animals that might actually have consciousness like a human," Newman says. "It might have human-type needs. We don't really know."

That possibility raises new questions about the morality of using the animals for experimentation. Another concern is that the stem cells could form human sperm and human eggs in the chimeras.

"If a male chimeric pig mated with a female chimeric pig, the result could be a human fetus developing in the uterus of that female chimera," Newman says. Another possibility is the animals could give birth to some kind of part-human, part-pig creature.

"One of the concerns that a lot of people have is that there's something sacrosanct about what it means to be human expressed in our DNA," says Jason Robert, a bioethicist at Arizona State University. "And that by inserting that into other animals and giving those other animals potentially some of the capacities of humans that this could be a kind of violation — a kind of, maybe, even a playing God."

Ross defends what his work. "I don't consider that we're playing God or even close to that," Ross says. "We're just trying to use the technologies that we have developed to improve peoples' life."

Still, Ross acknowledges the concerns. So he's moving very carefully, he says. For example, he's only letting the chimera embryos develop for 28 days. At that point, he removes the embryos and dissects them.

If he discovers the stem cells are going to the wrong places in the embryos, he says he can take steps to stop that from happening. In addition, he'd make sure adult chimeras are never allowed to mate, he says.

"We're very aware and sensitive to the ethical concerns," he says. "One of the reasons we're doing this research the way we're doing it is because we want to provide scientific information to inform those concerns."

Ross is working with Juan Carlos Izpisua Belmonte from the Salk Intitute for Biological Studies in La Jolla, Calif., and Hiromitsu Nakauchi at Stanford University.Daniel Garry of the University of Minnesota and colleagues are conducting similar work. The research is funded in part by the Defense Department and the California Institute for Regenerative Medicine (CIRM).

(
npr.org)

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Luis Miguel Goitizolo

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RE: ARE WE NOW IN THE END TIMES?
5/19/2016 5:53:47 PM

Sand storm buries 16 villages in southeastern Iran

May 18, 201

TEHRAN — 16 villages in Rigan, southeastern province of Kerman, were buried in sand and became completely deserted, IRIB reported.

Massive sand influx and consistent sand storms led to the complete disappearance of the villages under piles of sand, the Rigan governor, Amin Baqeri said.

Agriculture and livestock are totally ruined in this area and it suffered a loss of 320 billion rials (nearly $9 million), Baqeri added.

The continuance of sand influx has already endangered 80 other villages and if not stopped timely they are subjected to be deserted too, the governor warned.

MQ/MG

(tehrantimes.com)


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Luis Miguel Goitizolo

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RE: ARE WE NOW IN THE END TIMES?
5/19/2016 6:09:38 PM

Health officials to weigh declaring global emergency as yellow fever strikes southwest Africa


Ann M. Simmons

Alarmed that an outbreak of yellow fever in southwest Africa could spread if not quickly contained, medical experts will convene this week to consider whether to declare an international health emergency.

Though the panel convened by the World Health Organization may not make the declaration -- a move taken with Ebola and Zika outbreaks -- the session to be held in Geneva on Thursday speaks to the seriousness of the disease's spread in Angola. Yellow fever infections tied to Angola already have been reported in China.

"In my view calling an emergency committee for yellow fever is clearly the right thing to do," Lawrence Gostin, faculty director of Georgetown University's O'Neill Institute for National and Global Health Law, said in an email.

"First, there is the potential for rapid spread to other countries and regions, threatening the health of large populations in Africa, Asia and elsewhere," Gostin said. "Second, as the crisis escalates, global supplies of the yellow fever vaccine are dwindling and we could easily face a critical shortage. What this new emergency committee demonstrates is that mosquito-borne diseases — Zika and now yellow fever — pose major threats. This requires a war against mosquito vectors with resources and a full range of technologies, as well as health education."

The looming fear is a yellow fever outbreak in
Asia.

Meanwhile, the disease has been imported by travelers from Angola to Kenya, China and the Democratic Republic of Congo, raising the alarm in other nations, including Namibia and Zambia that share a long border with Angola. An unrelated bout of yellow fever has also taken hold in Uganda.

“The risks for other countries really depend on how fast the cases are detected and the density of mosquitoes,” Sylvie Briand, WHO's director of the pandemic and epidemic diseases department, told reporters at a briefing in Geneva last week. “We are concerned for other countries that may have high density of mosquitoes.”

Yellow fever is transmitted by Aedes aegypti mosquitoes, which also carry Zika, the virus linked to severe birth defects that have swept South America in recent months.

Patients with severe infection from yellow fever experience high temperatures, jaundice, bleeding and eventually shock and failure of multiple organs. Between 20% to 50% of those who become jaundiced, entering what is known as the “yellow” phase of the disease, die from the virus, WHO officials said.

The outbreak in Angola is particularly worrisome because it is happening in urban areas, such as the capital city Luanda, where the first cases in the country were detected, health officials said.

"You have trillions of mosquitoes and millions of people, so the capacity of transmission of the virus is multiplied enormously,” said Briand. The virus is far easier to fight in sparsely populated rural areas, she said.

While there is no treatment for yellow fever, the vaccination used to prevent the disease is highly effective. But the global supply of the vaccine is limited, according to information published WHO. The agency said that because of the current outbreak, shipments of the vaccine that would ordinarily be used in routine immunization programs in other countries where yellow fever is endemic were being used in Angola and other affected countries.

Even before the yellow fever outbreak, the emergence of other infectious diseases, such as Zika, has prompted some health specialists to call for overhauling how to respond to potential global health threats.

Gostin and Lucey are among health specialists and academics who are calling for the creation of a permanent committee that would devise a “quick and effective” plan of action and respond as soon as new threats emerge.

Prior delays in convening such committees for the Ebola virus, and possibly the ongoing Zika epidemic, “cost lives and should not be repeated,” the academics wrote in their recent paper.

An explosion of yellow fever is not farfetched given the challenges of controlling the spread of the virus. While more than 105 million Africans have been vaccinated since 2006 when WHO launched a yellow fever initiative, several African nations still are not completely protected against the disease.

Combating yellow fever is made more challenging because victims don't show definitive symptoms until the disease has progressed, making early detection difficult.

Such was the case in Angola where an infected person was initially thought to have died of food poisoning after eating at an open-air marketplace restaurant. Two or three days later, a few more people, friends of the first victim, also died. They had all eaten at the same restaurants, so food poisoning was again suspected. Subsequent tests done at a lab outside Angola revealed yellow fever, Briand said. The owner of the restaurant, which reportedly had many mosquitoes, also died.

Llanos Ortiz, deputy desk manager for the emergency unit of the medical humanitarian organization Doctors Without Borders, said she was particularly concerned about the infection spreading in places such as Kinshasa, capital of the Democratic Republic of Congo, with its teeming population, high number of mosquitoes and access to travel to other regions and countries.

“Those urban settings are very well connected with the rest of the world,” said Briand of WHO. “Unfortunately, many people don’t take seriously enough the issue of vaccinations for travelers. This is why we have had many cases exported.”

“The looming fear is a yellow fever outbreak in Asia,” where the disease is not currently endemic, said Lucey, the Georgetown immunologist.

China has reported 11 yellow fever cases of individuals infected in Angola, according to WHO.

Ray Arthur, director of the Global Disease Detection Operations Center at the Centers for Disease Control and Prevention, said in emailed comments that there had been no local transmission of yellow fever in the U.S. since the early 1900s. And while many areas in the country have mosquitoes that can become infected and transmit yellow fever virus, the agency was “not expecting local transmission of yellow fever in the continental United States, ” Arthur said.

However, the CDC is “concerned about travelers going to Africa and South America,” where yellow fever is endemic, Arthur said. “Travelers can return to the United States with a yellow fever virus infection.”

The agency is advising travelers to countries with yellow fever to review the vaccine recommendations and requirements to determine their need for vaccinations.

Copyright © 2016, Los Angeles Times

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Luis Miguel Goitizolo

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5/19/2016 6:38:44 PM

Venezuela is falling apart and its military is terrifying people



Venezuelan soldiers outside a supermarket while people lining up to buy basic food items in Caracas, Venezuela, on Sunday.


A week ago residents of the poorer barrios of Venezuela's capital, Caracas, woke up to find soldiers everywhere.

Dressed in black helmets, military fatigues, and bulletproof jackets, a fleet of soldiers on 400 motorcycles flew through narrow, crumbling, streets while being supported by trucks and two helicopters.

They set up street checkpoints and positioned themselves on rooftops to monitor the neighborhoods, while officers scoured alleys and houses holding heavy weaponry.

The military deployment took place in the midst of Venezuela's massive economic crisis triggered by the fall of oil prices and characterized by a shrinking economy and hyperinflation. Blackouts are now constant, in many areas water is severely rationed, and public-sector employees work just two days a week in an effort to save energy.

Patients are dying in hospitals for lack of basic care, desperate families are buying bootleg medication at inflated prices, and standing in huge lines is required to buy basic goods such as toilet paper, condoms, and rice.

At the same time President Nicolás Maduro faces a drive by the political opposition, emboldened by its electoral win in December that gave it control of congress for the first time in 17 years, to force a recall referendum.

The situation is so tense that last week's show of military strength sparked immediate rumors of a coup. What was actually happening was close to the opposite — the launch of a new phase of an anticrime offensive that some observers see as an effort by Maduro to show the unsettled population that he is still in charge and intends to remain so.

The Operation Liberation and Protection, or OLP, began in July. It is the country's 23rd anticrime initiative since President Hugo Chávez took office and launched his Bolivarian revolution in 1999. It is the third since his protegé, Maduro, began his term after Chávez died in 2013.

Reuters/Carlos Garcia RawlinsPeople trying to buy toilet paper outside a pharmacy in Caracas on Monday.

But, some say, anticrime operations in Venezuela are not necessarily primarily about the rampant criminal gangs that caused Caracas to be named the most violent city in the world in 2015 with a terrifying homicide rate of nearly 120 murders per 100,000 citizens.

"It's a preventative measure in the face of widespread dissatisfaction in Venezuela," criminal lawyer and well-known criminologist Luis Izquiel said. "People want to see a political change with the referendum, because they are hungry and lack most things."

The new stage of the OLP was announced with great fanfare on state-owned TV. Reports detailed the number of soldiers, cars, ambulances, tanks, and helicopters involved, as well as the exact details of the equipment they carried.

When the OLP was first launched in July, it promised to dismantle criminal gangs operating in poor barrios. It consisted of military sweeps that caught up anyone who was allegedly involved and, critics say, many others who were not.

Izquiel, the lawyer, says the second stage of the OLP appears more directed toward political control, with the military now preparing to occupy some areas for longer periods of time.

"Here you can see that the message is the intimidation," he said. "They are going to stay for some time to prevent public dissatisfaction from showing."

Even if the aim is to control crime, critics argue that the OLP should be discontinued because it has both failed to reduce homicide rates — which increased by 7% in the first few months of this year compared with the same period in 2015 — and encourages abuse.

"It's only good for violating human rights and criminalizing poverty," Izquiel said. "When the OLP targets a poor area, it destroys anything that stands in its way, tearing down houses."

Reuters/Marco BelloAn opposition supporter shouting toward riot police officers in a rally to demand a referendum to remove President Nicolás Maduro, in Caracas on May 11.

A report released last month by a local human-rights group called Provea, in association with the US-based Human Rights Watch, detailed extrajudicial executions, arbitrary detentions, forced evacuations, and the destruction of many homes.

Irene González tells the story of how she lost her house to the OLP last year, and went on to form the Committee of Victims from Ensenada, a poor area in the state of Miranda.

"I woke up early to go to work, around 4 a.m.," she said. "It was raining. I saw a lot of soldiers as I left my home. Then I found out there were 1,500 of them. They told me I could not go out, because it was an OLP. I asked how long it would take, as I had to go to work, and their response made me shiver because they said 'For as long as it takes us to tear down your house.'"

González said the operation led to the capture of two people who were later released, and the formation of the committee of victims by 110 families whose homes were destroyed by what they say they were told was a presidential order.

"There's no excuse to leave all those families in the street," González said. "There were no criminals or drugs in the area."

Perhaps most worrying for the government is that González is not somebody who has been easily turned against the "socialist" project launched by Chávez 17 years ago and is now under the charge of Maduro.

"I'm a Chavista, but I don't like Maduro," she said. "Our beloved president Chávez would have never allowed this atrocity to take place."

Reuters/Carlos Garcia RawlinsMaduro at a news conference at Miraflores Palace in Caracas on Tuesday.

The recently implemented second stage of the OLP included an initial claim of success, in the form of the capture and death of José Antonio Tovar Colina, also known as El Picure, who was said to head a criminal group that controls three states.

Jamilton Andrés Suárez Ulloa, also known as El Topo, was also killed in the operation. He reportedly controlled the mining areas in the Bolívar state and was the alleged mastermind behind the death of a group of miners in Tumeremo earlier this year.

"There's a society that is demanding safety after being overwhelmed by criminal leaders," said Inti Rodríguez, member of the human-rights groups Provea, that helped elaborate last month's report. "And the government is answering with the same heavy-handed policy that enables it to say it's doing something."

Rodríguez, however, added that this time the political side to the operation was particularly obvious.

"There's a clear motive behind the launch of the second stage of the OLP. The referendum to revoke Maduro's presidency is on the horizon," he said. "The security operation has a huge propaganda effect and the militarization of poor areas stops an uprising from taking place."

The relaunched OLP came three days before president Maduro declared a 60-day state of emergency that, if approved by the national legislature, would give him wider powers of state intervention in the economy, which are already considerable.

Maduro, meanwhile, has stepped up his already frequent allegations that an internationally backed intervention or coup against him is imminent. This Saturday he told a large gathering of supporters that the military will start exercises in a week in order to ensure it is prepared for "whatever scenario."

Read the original article on VICE News. Check them out on YouTube, Facebook, andInstagram. Copyright 2016. Follow VICE News on Twitter.

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Luis Miguel Goitizolo

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RE: ARE WE NOW IN THE END TIMES?
5/20/2016 12:54:30 AM

Nigeria confirms rescue of one of the kidnapped Chibok girls

Quartz


More than two years after the abduction of more than 200 schoolgirls by militant sect Boko Haram in Chibok, a town in Nigeria’s north east, the Nigerian army says one of the girls has been rescued.

The girl, Amina Ali, was found by a local vigilante group in Sambisa Forest in the northeastern state of Borno—a known base for Boko Haram operations. Her parents later confirmed her identity as one of the kidnapped girls. Amina reportedly confirmed that other girls were still in the custody of Boko Haram but that as many as six have died in captivity.

The kidnap of the girls gained global attention in April 2014 with the high profile Bring Back Our Girls campaign, as the Nigerian government came under intense criticism for its initial lax response to the abduction. Since then, there has been speculation about the whereabouts of the girls with various reports suggesting they had been married off to Boko Haram fighters or sold to other militia groups in neighboring countries.

The Nigerian government has previously indicated an interest in negotiating with the sect to secure the release of the girls. The group reportedly demanded a $50 million ransom for the release of the girls and released a “proof of life” video showing some of the abducted girls to seemingly facilitate a negotiation.

In the last year, under Nigerian president Muhammadu Buhari, the Nigerian army has recorded strong gains against Boko Haram, retaking territory and rescuing abducted women and girls.


(Yahoo News)


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